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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was located to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential function in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two extra splice variants were being described but thorough nucleotide sequence information will not be however out there.

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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope identified through the antibody.

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This information outlines further achievable optimization of mobile and immunoassay parameters to be sure the very best outcomes are...

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This information outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.

Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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